Disease context
Asthma
Airway inflammatory disease
- Stimulus
- House dust mite extract
- Experimental context
- 16HBE + HDM
- Stimulus parameters
- dose: NOT_REPORTED; exposure duration: NOT_REPORTED
Application purpose
- Asthma-related epithelial inflammatory response
Evidence summary: Directly supports 16HBE14o- (the canonical entity for the 16HBE query) as an in-vitro HDM airway-inflammation application context. The human airway epithelial line 16HBE14o- was exposed to house dust mite and assessed for CCL20 secretion and signaling pathways; findings were compared with primary bronchial cells. Supports HDM-driven barrier and inflammatory-response readouts in 16HBE airway epithelium. HDM effects on calcium signaling, barrier function and CCL20 release were tested in human bronchial epithelial 16HBE cells alongside primary bronchial epithelial cells.
Interpretation: Used to study HDM-associated bronchial epithelial inflammatory and barrier responses in 16HBE14o-.
Limitation: An immortalized line and a defined chemokine/signaling endpoint do not model all asthma biology or establish disease fidelity. The cell line is an immortalized bronchial model and cannot capture donor heterogeneity or the full asthma airway environment.
Supporting references
- PMID 19178937 — Innate immune responses of airway epithelium to house dust mite are mediated through beta-glucan-dependent pathways
- PMID 23915187 — House dust mite-induced calcium signaling instigates epithelial barrier dysfunction and CCL20 production